Material y sedoanalgesia en el procedimiento de punción lumbar neonatal / Material and sedation-analgesia in the neonatal lumbar puncture procedure

Introducción: La punción lumbar (PL) es un procedimiento que se realiza con frecuencia durante el período neonatal. Efectuar correctamente esta técnica puede evitar gran parte de sus complicaciones. Objetivo: Evaluar el procedimiento de PL en neonatos en los hospitales españoles, estudiando el tipo de material empleado y la sedoanalgesia utilizada. Material y métodos: Estudio transversal basado en una encuesta escrita distribuida por correo electrónico a través de la Red de Hospitales Segurneo-SEN y la Sociedad Española de Neonatología, que recogía datos sobre la técnica de PL en neonatos. Resultados: Se analizaron 301 encuestas respondidas. La mayoría de profesionales utilizaban aguja tipo trocar con estilete como primera opción (89,7%). No obstante, cuando la PL era fallida o hemática el 32,2% cambiaba el tipo de aguja. Del total de médicos, 143 reflejaron su sensación subjetiva sobre el tipo de aguja: el 41,3% solo empleaban agujas tipo trocar, al 32,2% la técnica les resultaba más fácil empleando agujas de tipo «palomilla», para el 7,7% existía menor probabilidad de PL hemática o fallida usando agujas de tipo «palomilla», el 10,5% elegían aguja dependiendo del tamaño del neonato, y un 8,4% eran indiferentes a utilizar un tipo u otro de aguja. El 99% de los encuestados usaban algún tipo de analgesia. Las medidas más utilizadas fueron anestésicos tópicos (90,3%) y sacarosa (82,2%). Conclusiones: Actualmente, el procedimiento de PL en neonatos se realiza de forma mayoritaria utilizando agujas tipo trocar con estilete y con un uso extendido de diferentes medidas analgésicas para llevar a cabo la técnica. (AU)

Introduction: Lumbar puncture (LP) is a frequent procedure during the neonatal period. Correctly performing this technique can avoid many of its complications. Objective: To evaluate the LP procedure in neonates in Spanish hospitals, studying the type of material and the sedation-analgesia used. Material and methods: Cross-sectional study conducted in Spain gathering data through a survey research distributed by email through the Segurneo-SEN Hospital Network and the Spanish Neonatal Society. Data on the LP technique in neonates were collected. Results: A total of 301 participants were analysed. Most professionals used a trocar needle with a stylet as the first option (89.7%); however, when the LP failed or was blood-stained, 32.2% changed the type of needle. A total of 143 doctors gave their subjective feeling about the type of needle: they only use trocar needles with stylet (41.3%), the technique is easier using scalp-vein needles (32.2%), there is less probability of a blood-stained LP, or failed using scalp-vein needles (7.7%), they choose the type of needle depending on the size of the new-born (10.5%), indifference when using one type or another needle (8.4%). Almost all (99%) of the surveyed doctors used some type of analgesia. The most widely used measures were topical anaesthetics (90.3%) and sucrose (82.2%). Conclusions: Currently, the LP procedure in the neonatal period is mainly performed with a trocar needle with a stylet and with the widespread use of different analgesic measures. (AU)

Emergency physician performed ultrasound-assisted lumbar puncture in children: A randomized controlled trial.

BACKGROUND AND OBJECTIVE: Lumbar puncture (LP) is one of the most common procedures performed in pediatric emergency departments but first-attempt success rates remain low and traumatic LP remains frequent. The aim of this study was to determine if ultrasound performed by emergency physicians improves the probability of first-attempt success for lumbar puncture in children. METHODS: This was a prospective randomized controlled trial conducted in two tertiary care, pediatric, university-affiliated emergency departments in 2017-2018. Eligible participants were children younger than 19 years old requiring an LP in the emergency department. They were randomized to either the standard landmark-based LP (SLP) or ultrasound-assisted LP (UALP) groups. Our primary outcome was the first-attempt LP success rate. RESULTS: 166 patients were enrolled, with 84 in the UALP and 82 in the SLP group. A total of 23 physicians performed ultrasounds in the study. The proportion of successful first-attempt LP was higher in the ultrasound group (60/84; 68%) than for the standard procedure (52/82; 60%), but this failed to reach statistical significance (difference: 8.1%; 95% CI: -6.4 to 22.2). CONCLUSION: Our study does not support the routine use of ultrasound for performing lumbar puncture in children. Considering the minimal harm and ease of performance, ultrasound may be used as an adjunct and teaching tool in certain clinical situations.

Low Incidence of Postdural Puncture Headache Further Reduced With Atraumatic Spinal Needle: A Retrospective Cohort Study.

BACKGROUND: The purpose of the study was to evaluate the incidence of postdural puncture headache in a predominantly pediatric sample before and after a transition from conventional to atraumatic spinal needles. METHODS: In this retrospective cohort study, we analyzed data from 1059 lumbar puncture procedures in 181 individuals enrolled in NIH Clinical Center research protocols. Multivariate logistic regression was used to evaluate the association between postdural puncture headache and spinal needle type after adjusting for patient age, sex, and body mass index. A random effect of participant was used to accommodate repeated observations. RESULTS: The median age at time of procedure was 15.3 years. The overall rate of postdural puncture headache was 5.1% (54 of 1059). With conventional needles and atraumatic needles, respectively, the rate of postdural puncture headache was 7.7% (43 of 588) and 2.3% (11 of 471); (odds ratio 0.32, 95% confidence interval 0.15 to 0.68). CONCLUSIONS: Lumbar puncture for cerebrospinal fluid collection is an essential and common procedure in pediatric clinical care and research. Postdural puncture headache is the most common adverse event of the lumbar puncture procedure. Our data indicate that lumbar puncture is safe in pediatrics and that use of an atraumatic spinal needle further reduces the risk of postdural puncture headache.

Risk of intervertebral disc joint puncture during lumbar puncture.

OBJECTIVE: Penetration of intervertebral disc joint during lumbar puncture might be unnoticed during procedure. However, accelerated degeneration of the disc joint is a long-term consequence of inadvertent penetration. In this paper, we aimed to demonstrate and evaluate the risk of disc puncture during standard lumbar puncture. PATIENTS AND METHODS: 50 human cadavers were used in this study. Disc puncture risk was assessed by using worst case scenario model. Lumbar puncture was performed in a standard fashion using midline route at L3-4, L4-5 and L5-S1 levels. The needle was advanced until it hit resistance from the bone. Lateral X-ray was used to visualize the needle position. Needle ended up in two possible locations - posterior vertebral body wall and intervertebral disc space. RESULTS: The probability of puncturing the joint was 20 % for L3-4, 38 % for L4-5, 16 % for L5-S1. Total probability of disc penetration was 25 %. Statistical analysis revealed significantly increased risk for performing LP at L4-5 level in comparison with L5-S1 (p = 0.023). CONCLUSION: Lumbar puncture carries significant risk of intervertebral disc penetration. This complication is not realized during the procedure and lead to accelerated joint degeneration.

Spinal needle size and traumatic neonatal lumbar puncture: an observational study (neo-LP).

The incidence of 'traumatic' lumbar puncture (LP; CSF red cells > 500/mm) has been reported to be 35-46% in the neonatal period. A traumatic LP incurs challenges in diagnosis and management of the underlying condition and increases the risk of complications. We aimed to assess the benefits of a smaller outer diameter, larger gauge 25G needle in reducing the incidence of traumatic LPs compared with the standard 22G LP needle. This prospective observational study compared data from two consecutive epochs. Epoch 1 (Control, April 2016-October 2016), 22G needle for LP as standard practice. Epoch 2 (Intervention, November 2016-October 2017) 25G needle used for LP. Primary outcome was the incidence of traumatic LP. Multiple logistic regression analyses were conducted adjusting for corrected gestational age (CGA) at LP, proceduralist experience and need for ventilation as an indicator of illness. There were 240 LPs during the study period involving 361 attempts (22G, n = 228; 25G, n = 133). Median gestation at birth (P = 0.617) and CGA at LP (P = 0.163) were comparable. Multivariate analysis revealed lower incidence of traumatic LP using 25G needle (P < 0.001). Incidence of obtaining a successful CSF sample was similar between groups (P = 0.944). Proceduralist experience (P = 0.189) and neonatal illness (P = 0.801) were not significant factors.Conclusion: Our results showed that traumatic LPs were ~ 50% less common with 25G vs 22G needles while retaining a comparable success rate. Dry taps were more likely among the 25G group.What is Known:• The incidence of neonatal 'traumatic' lumbar puncture (CSF red cells > 500/mm) has been reported to be 35-46%.• A traumatic lumbar puncture incurs challenges in diagnosis and management of the underlying condition and increases the risk of complications.What is New:• Multivariate analysis revealed lower incidence of traumatic lumbar puncture using 25G needle (vs 22G).• Incidence of obtaining a successful CSF sample was similar between groups.

Prospective randomized comparison of cerebrospinal fluid aspiration and conventional popping methods using 27-gauge spinal needles in patients undergoing spinal anaesthesia.

BACKGROUND: Performing spinal anaesthesia using the conventional popping method with a 27-gauge (27G) spinal needle is technically difficult. In this study, we compared the aspiration and conventional popping method for spinal anaesthesia using 27G Quincke-type needles. METHODS: This prospective, randomized study enrolled 90 patients, aged 19 to 65 years, with American Society of Anesthesiologists physical status I-III, who were undergoing spinal anaesthesia. Patients were randomly assigned to one of two groups using a computer-generated random number table: patients receiving spinal anaesthesia using the aspiration method, in which the needle is advanced with continuous aspiration, or the conventional popping method. The primary outcome measure was the success rate of the first attempt to perform dural puncture. Number of attempts and passages, withdrawal cases, successful attempt time, total procedure time, and actual depth of dural puncture were recorded. RESULTS: Eighty-eight patients were included in the study. In the aspiration group, the success rate of first attempt for dural puncture was 93.3%, compared with 72.1% in the popping group (P = 0.019). Success involving needle withdrawal was recorded in 4 (8.9%) patients in the aspiration group and 13 (30.2%) in the popping group (P = 0.024). In the popping group, the number of attempts was significantly higher (P = 0.044), and total procedure time was significantly longer (P = 0.023). Actual depths of dural puncture were deeper in the popping group than in the aspiration group (P = 0.019). CONCLUSIONS: The aspiration method using a 27G Quincke-type needle offers clinical benefits for dural puncture compared with the conventional popping method for spinal anaesthesia. TRIAL REGISTRATION: Clinical research information service number: KCT0002815, registered 21/Apr/2018. Retrospectively registered.

Encefalitis herpética / Herpes encephalitis

No disponible

Cefalea postpunción dural con aguja espinal de bisel cortante aguja espinal de punta cónica: revisión sistemática / Postdural puncture headache with cutting spinal needle pencil point spinal needle: systematic review

INTRODUCTION: Postdural puncture headache is one of the most frequent complications that occurs after the puncture of the dura mater, resulting in the spinal fluid leakage, through the same and subsequent traction of brain structures. OBJECTIVE: To compare cutting spinal needle and pencil point spinal needle in the incidence of postural dural puncture headache. MATERIALS AND METHODS: A literature search of scientific articles published since 2006 was carried out in the Pubmed, Science Direct, Clinical Key and EBSCO databases, which evaluates the incidence of postdural puncture headache in spinal needles with conical tip and cutting bevel, additionally, experts will be consulted in the area that should suggest literature related to this topic, which would not have been included in the search methods previously described. RESULTS: In total, 14 studies were selected in the population that suffered puncture of the dura mater after accidental, therapeutic or diagnostic lumbar puncture (LP) and the presentation of postdural puncture headache was measured according to the ICHD-3 beta criteria. It was found that there is a decrease in the incidence of postdural puncture headache with conical tip spinal needles, compared to spinal needles of cutting bevel. CONCLUSIONS: Current evidence indicates that postdural puncture headache occurs less frequently, with low intensity and short duration in patients operated with a conical spinal needle, compared to the cutting bevel. In addition, according to the recently described pathophysiology, it is contradictory that conical spinal needles are called "atraumatic", so this review proposes a change in nomenclature that is most useful for the clinical anesthesiologist.

INTRODUCCIÓN: La cefalea postpunción dural es una de las complicaciones más frecuentes que se presenta posterior a la punción de la duramadre, lo cual produce salida de líquido cefalorraquídeo, a través de la misma y posterior tracción de las estructuras cerebrales. OBJETIVO: Comparar la aguja espinal de bisel cortante y la aguja espinal de punta cónica en la incidencia de presentación de cefalea postpunción dural. MATERIALES Y MÉTODOS: Se realizó una búsqueda bibliográfica de artículos científicos publicados desde el año 2006, en las bases de datos Pubmed, Science Direct, Clinical Key y EBSCO, que evaluaran la incidencia de cefalea postpunción dural en agujas espinales de punta cónica y bisel cortante, adicionalmente se consultó con expertos en el área que pudieran sugerir literatura relacionada con este tema, la cual no hubiese sido incluida en los métodos de búsqueda previamente descritos. RESULTADOS: En total se seleccionaron 14 estudios realizados en población que sufrieron punción de la duramadre luego de punción lumbar (PL) accidental, terapéutica o diagnóstica y se midió la presentación de cefalea postpunción dural según los criterios ICHD-3 beta. Se encontró que hay una disminución de la incidencia de cefalea postpunción dural con las agujas espinales de punta cónica, en comparación con las agujas espinales de bisel cortante. CONCLUSIONES: La evidencia actual indica que la cefalea postpunción dural se presenta con menor frecuencia, baja intensidad y corta duración en pacientes intervenidos con aguja espinal de punta cónica, en comparación con la aguja espinal de bisel cortante. Adicionalmente, según la fisiopatologia recientemente descrita, es contradictorio que las agujas espinales de punta cónica sean llamada "atraumáticas", por lo que esta revisión propone un cambio en la nomenclatura que resulta de mayor utilidad para el anestesiólogo clínico.

Effects of stylet-in versus stylet-out collection of cerebrospinal fluid from the cisterna magna on contamination of samples, sample quality, and collection time.

OBJECTIVE: To evaluate safety of stylet-in and stylet-out techniques for collection of CSF from the cisterna magna and to assess whether there were differences between techniques with regard to contamination of samples, sample quality, and efficiency of collection. ANIMALS: 10 adult purpose-bred research Beagles. PROCEDURES: A prospective crossover study was conducted. Preanesthetic physical and neurologic examinations and hematologic analyses were performed. Dogs were anesthetized, and collection of CSF samples from the cisterna magna by use of a stylet-in or stylet-out technique was performed. Two weeks later, samples were collected with the other sample collection technique. Samples of CSF were processed within 1 hour after collection. RESULTS: Cellular debris was detected in higher numbers in stylet-in samples, although this did not affect sample quality. The stylet-out technique was performed more rapidly. No adverse effects were detected for either technique. CONCLUSIONS AND CLINICAL RELEVANCE: Both techniques could be safely performed in healthy anesthetized dogs. The stylet-out technique was performed more rapidly and yielded a sample with less cellular debris. Both techniques can be used in clinical practice to yield CSF samples with good diagnostic quality.

Multicentre clinical simulation evaluation of the ISO 80369-6 neuraxial non-Luer connector.

To avoid potentially fatal wrong-route neuraxial drug errors, international standard ISO 80369-6 specifying a non-Luer neuraxial connector design was published in 2016. We describe usability studies used in development of the design. Thirty-eight doctors and 17 nurses performed simulated procedures on manikins, using devices fitted with Luer connectors or draft ISO 80369-6 'non-Luer' connectors. The procedures included spinal anaesthesia; intrathecal chemotherapy; lumbar puncture, cerebrospinal fluid collection and pressure measurement; epidural catheter placement with bolus injection and critical care use. Participants attempted cross connection between neuraxial connectors and a range of other medical device connectors, including those from the ISO 80369 small-bore connector series. Video recording analysis was used for all assessments. Participants subjectively assessed performance of the draft non-Luer connector, including suitability for routine clinical use. Participants performed 198 procedures. The connector achieved easy, leak-free connections. The willingness of participants to use the non-Luer connectors were: spinal anaesthesia 100%; intrathecal chemotherapy 88%; lumbar puncture, cerebrospinal fluid collection and pressure measurement 93%; epidural catheter placement with bolus injection 78%; critical care use 100%. Concerns raised were generally device related, rather than connector related. Most cross-connection attempts failed, even using above clinical forces and, when successful, were judged of low clinical risk potential; the exception was a malaligned connection between the non-Luer slip and female Luer connectors. This led to revision of the dimensional tolerances of the non-Luer connector to reduce this risk, before publication of the final specification in 2016. We conclude that the ISO 80369-6 neuraxial non-Luer connector is suitable for clinical use.

Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice.

Delivery of viral vectors directly into the central nervous system (CNS) has emerged as an important tool for the refinement of gene therapy. Intrathecal delivery by direct lumbar puncture in conscious rodents offers a minimally invasive approach that avoids tissue damage and/or destruction. Here we describe delivery of small quantities of viral vector product to the intrathecal space of rodents via direct lumbar puncture aided by a catheter.

Encefalomielitis extensa por Borrelia: una forma atípica de neuroborreliosis / Extensive encephalomyelitis due to Borrelia: an atypical form of neuroborreliosis

No disponible

Clinical practice guideline on atraumatic (pencil-point) vs conventional needles for lumbar puncture: Endorsement by the Scandinavian Society of Anaesthesiology and Intensive Care Medicine.

The Scandinavian Society of Anaesthesiology and Intensive Care Medicine Clinical Practice Committee endorses the BMJ Rapid Recommendation clinical practice guideline on atraumatic (pencil-point) vs conventional needles for lumbar puncture. This includes the strong recommendation for the use of atraumatic needles for lumbar puncture in all patients regardless of age or indication.

Cerebrospinal fluid collection in laboratory mice: Literature review and modified cisternal puncture method.

BACKGROUND: The composition of cerebrospinal fluid (CSF) is an invaluable parameter in better understanding of cellular and molecular processes within the mammalian brain. However, the collection of significant volumes of clean CSF can be technically challenging in studies with laboratory mice. Over the past five decades, several approaches have been developed to maximize the quantity and quality of CSF samples, either from live or euthanized animals. Due to the small amounts collected, samples from single mice were often pooled or diluted to meet volume requirements of automated counters and multiple assays. NEW METHOD: This paper reviews previous work on CSF collection in mice, thus providing methodological background for the current post-mortem procedure. This modified cisternal puncture method involves the use of a peristaltic pump for consistent and slow intracardiac perfusion, as well as a loupe headset with a custom-made glass pipette for piercing a single hole in the atlanto-occipital membrane during repeated CSF draws. Sample cleanness is verified by comparing the colour of the glass pipette and the bottom of centrifuged PCR vial against a white background. RESULTS: With three trained experimenters, the entire procedure (including anesthesia) takes ∼11-13 min and often results in the collection of up to 40 µl of clean CSF from males of different murine strains. Properly staggered collections allow processing of relatively large cohorts of mice per day. CONCLUSIONS: This modification of previously employed methods can be used in studies that require tightly-timed collections of larger volumes of undiluted, tissue-free CSF and/or individual data records.

A systematic review of DURAL puncture epidural analgesia for labor.

STUDY OBJECTIVE: This systematic review aimed to summarize the evidence derived from randomized controlled trials (RCTs) comparing dural puncture epidural analgesia (DPEA) and conventional lumbar epidural analgesia (LEA) for women undergoing labor. INTERVENTIONS: The MEDLINE and EMBASE databases were searched from inception to July 2018 in order to find RCTs published in the English language, which investigated DPEA in laboring women. MAIN RESULTS: Six RCTs were included in the final analysis. Their collective results remain ambiguous. Dural puncture with small (i.e., 26- or 27-gauge) spinal needles seems to confer either minimal benefits or improved analgesic quality and lower pain scores in the first 10 min. Dural puncture with 25-gauge spinal needles has been reported to provide higher success rate than conventional LEA in one trial; however two other studies could only agree on the fact that DPEA results in improved sacral blockade and fewer unilateral blocks compared to LEA. CONCLUSIONS: The current evidence regarding DPEA for labor analgesia remains ambiguous. Future research should investigate the optimal (spinal) needle size for dural puncture as well as factors governing transmeningeal flux of local anesthetics and opioids in the presence of a dural hole.